Balance Beam

         The balance beam task consists of a beam (117x28.5x2 cm), which is suspended 100 cm over a 7 cm thick foam cushion, and a goal box (24.5x20x18 cm).  Animals are trained on a balance beam six times, consisting of 2 trials per day for 3 consecutive days prior to surgery.  Familiarity with the task is imperative so mistakes are caused from deficits alone.  Mistakes are quantified as foot slips. Then the rats are to be tested for a pre-surgical run on the balance beam.  Following surgery, animals will be tested one week following surgery and prior to retention in the RAWM.  Latency to begin movement and the number of foot-slips will be recorded.  Latency to begin movement may be defined as the time between placement of the animal on the beam and when the animal crosses the 17 cm mark from the end of the beam.  Foot-slips occur when a paw descends lower than a line drawn 1.5cm below the surface of the beam, which runs along the length of both sides of the beam.  Foot-slips are recorded after the front paws of the animal cross the preset mark until the nose enters the goal box.  The task must be completed in 3 minutes.  The task is considered incomplete if the animal exceeds three minutes, or falls off the beam