TBS 0.05M

 

1.   Label flask: date/time/contents

2.   Weigh

a.   9 g NaCl

b.   12.1 g Triz base

 

3.   Add above to 1000 ml dH2O and stir

4.   pH to:

a.   8.0, NADPH

b.   7.3, immunostaining