Perfusing
v
Gloves + Mask + Goggles (all people in
room)
Anesthetization
1.
Prepare 1 ml syringe with 1 ml sodium pentobarbital
(Nebutal)
· Remove any
air in syringe
2.
10.
Start pumping fixative:
~ 500 ml
11.
Carefully remove cannula and release animal from clamps